aroC [2019-08-07 08:55:40]

3-dehydroquinate dehydratase

locus: BSU_23080

pI: 4.90

mw: 27.98 kDa

protein length: 255 aa

gene length: 768 bp

function: biosynthesis of aromatic amino acids

product: 3-dehydroquinate dehydratase

essential: no

ec: 4.2.1.10

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.13|Biosynthesis/ acquisition of aromatic amino acids]

Gene

Coordinates: 2,412,706 2,413,473

The protein

Catalyzed reaction/ biological activity

• 3-dehydroquinate = 3-dehydroshikimate + H₂O (according to Swiss-Prot)

Protein family

• type-I 3-dehydroquinase family (single member, according to UniProt)

Structure

• [PDB|1GQN] (from ''Salmonella typhi'', 55% identity, 70% similarity) [Pubmed|11976491]

Expression and Regulation

Operons

• genes: [gene|61EA842B400548A99504A40467796B983DD54634|aroC]
  description: [Pubmed|7934830]

Biological materials

Mutant

• BKE23080 ([gene|61EA842B400548A99504A40467796B983DD54634|aroC]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE23080 BGSC] and in [SW|Jörg Stülke]'s lab, [Pubmed|28189581], upstream reverse: _UP1_CACTCGTCTCAAATCCTTTC, downstream forward: _UP4_TAGAACAATAAAAAAACTCA
• BKK23080 ([gene|61EA842B400548A99504A40467796B983DD54634|aroC]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK23080 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTCGTCTCAAATCCTTTC, downstream forward: _UP4_TAGAACAATAAAAAAACTCA

References

• 6442253,7934830